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| Effects of hydrogen sulfide on rats hypothalamic-pituitary- adrenal axis response during sepsis |
| Peng Chao, Qiao Wei, Pang Zhong-duo, Yue Jian-yun, Qi Ying-wen, Lu Yan, Chai Chen |
| Department of General Surgery, First Hospital of Lanzhou University, Lanzhou 730000, China |
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Abstract Objective To investigate the effects of hydrogen sulfide（H2S）on serum corticoste⁃ rone（CORT）, adrenocorticotropin（ACTH）and hypothalamic corticosterone（CRH）concentration in septic rats. Methods Sepsis was induced in adult male Sprague-Dawley（SD）rats by cecal ligation and puncture（CLP）. Sixty-three rats were randomly divided into nine groups: sham operation group; sepsis 6 h, 12 h, 24 h groups; CLP 12 h + sodium hydrosulfide（NaHS）; CLP 12 h+ hydroxylamine （HA）+ propargylglycine（PAG）group; CLP 12 h+ HA group; CLP 12 h+ PAG group（n＝7 in everygroup）. CLP only rats were sacrificed at 6 h, 12 h, 24 h after CLP. The others received intraperitoneal in⁃ jection of different drugs 1 h before CLP and were sacrificed at 12 h after CLP. The samples of serum or hypothalamus were collected. Serum CORT, ACTH and hypothalamic CRH concentration were detected by enzyme-linked immunosorbent assay（ELISA）. Results ①Serum CORT, ACTH and hypothalamic CRH concentrations increased significantly 6 h, 12 h, 24 h after CLP（all P＜0.05）. Serum CORT, ACTH and hypothalamic CRH concentrations were significantly higher 12 h, 24 h after CLP than that of 6 h after CLP（all P＜0.05）. Serum CORT concentration at 24 h after CLP was significantly higher than that 12 h after CLP（P＜0.05）, serum ACTH concentration was significantly lower than that 24 h after CLP（P＜0.05）. ②Inhibition of central or peripheral H2S generation increased serum CORT, ACTH and hypothalamic CRH concentrations（all P＜0.05）. Inhibition of the production of central H2S increased serum CORT, ACTH and hypothalamic CRH concentration（all P＜0.05）. Inhibition of the production of peripheral H2S increased serum CORT concentration（P＜0.05）and decreased hypothalamic CRH concentration（P＜0.05）. Serum ACTH concentration did not significantly changed（P＜0.05）. Conclusion HPA axis is active during sepsis; H2S inhibit HPA axis response by central and peripheral mechanism during sepsis.
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Received: 19 October 2015
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Corresponding Authors:
Chai Chen, E-mail: chasechai@126.com; Lu Yan, E-mail: lu73free@ aliyun.com
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